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two hybrid y2h bait vector pas2 1  (TaKaRa)


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    TaKaRa two hybrid y2h bait vector pas2 1
    Identification of I-2 domains required for yeast two-hybrid <t>(Y2H)</t> interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and <t>pAS2-1</t> empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.
    Two Hybrid Y2h Bait Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two hybrid y2h bait vector pas2 1/product/TaKaRa
    Average 96 stars, based on 609 article reviews
    two hybrid y2h bait vector pas2 1 - by Bioz Stars, 2026-04
    96/100 stars

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    1) Product Images from "Protein–protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2"

    Article Title: Protein–protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2

    Journal: Journal of Experimental Botany

    doi: 10.1093/jxb/ers021

    Identification of I-2 domains required for yeast two-hybrid (Y2H) interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and pAS2-1 empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.
    Figure Legend Snippet: Identification of I-2 domains required for yeast two-hybrid (Y2H) interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and pAS2-1 empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.

    Techniques Used: Construct, Activation Assay, Marker, Expressing, Western Blot, Binding Assay



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    two hybrid y2h bait vector pas2 1  (TaKaRa)
    96
    TaKaRa two hybrid y2h bait vector pas2 1
    Identification of I-2 domains required for yeast two-hybrid <t>(Y2H)</t> interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and <t>pAS2-1</t> empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.
    Two Hybrid Y2h Bait Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two hybrid y2h bait vector pas2 1/product/TaKaRa
    Average 96 stars, based on 1 article reviews
    two hybrid y2h bait vector pas2 1 - by Bioz Stars, 2026-04
    96/100 stars
    		  Buy from Supplier
    two hybrid bait vector pas2 1  (TaKaRa)
    96
    TaKaRa two hybrid bait vector pas2 1
    Identification of I-2 domains required for yeast two-hybrid <t>(Y2H)</t> interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and <t>pAS2-1</t> empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.
    Two Hybrid Bait Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/two hybrid bait vector pas2 1/product/TaKaRa
    Average 96 stars, based on 1 article reviews
    two hybrid bait vector pas2 1 - by Bioz Stars, 2026-04
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification of I-2 domains required for yeast two-hybrid (Y2H) interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and pAS2-1 empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.

    Journal: Journal of Experimental Botany

    Article Title: Protein–protein interactions as a proxy to monitor conformational changes and activation states of the tomato resistance protein I-2

    doi: 10.1093/jxb/ers021

    Figure Lengend Snippet: Identification of I-2 domains required for yeast two-hybrid (Y2H) interaction with I2I-1 and I2I-2. (A) Y2H analyses show interactions of I2I-1 or I2I-2 with full-length I-2 as bait, but not with Rx and Mi-1.2. I2I-1 and I2I-2 interact with the CC-NB-ARC bait of I-2 (amino acids 1–520), but not with bait constructs containing the CC-NB-ARC domains of I-2C1 (amino acids 1–526), Rx (amino acids 1–426), or Mi-1.2 (amino acids 161–896). The baits and preys did not show autoactivity when co-expressed with the pACT2 and pAS2-1 empty vectors. The test for activation of the HIS3 marker is shown after 10 days of growth (left panel). Expression of the bait proteins was confirmed by Western blot (WB) analyses using α-Gal4BD antibody detection on total yeast protein extracts (right panel). (B) Y2H analyses of I2I-1 and I2I-2 with various I-2 baits: I-2FL (full-length: amino acids 1–1266), I-2N+ (amino acids 1–643), I-2N (amino acids 1–520), I-2N ΔMHD (amino acids 1–520, with deletion at amino acids 484–496), and I-2CC (amino acids 1–170). The different I-2 (sub)domains are indicated and coloured: orange, coiled-coil (CC); red, nucleotide-binding (NB); purple, Apaf-1, R proteins, and Ced-4 (ARC) 1; blue, ARC2; and green, leucine-rich repeat (LRR). The test for activation of the ADE2 marker is shown after 5 days of growth; the other two markers ( HIS3, LacZ ) gave identical results. +, activation of all three selectable markers; –, no activation of these markers. The smallest part of I-2 enabling interaction with I2I-1 is the CC domain and for I2I-2 the CC-NB-ARC domain.

    Article Snippet: The bait used for the library screen was constructed by subcloning the Nco I/ Sac I fragment of I-2 FL in the yeast two-hybrid (Y2H) bait vector pAS2-1 (Clontech Laboratories, Palo Alto, CA, USA), resulting in bait CC-NB-ARC-LRR1-12 (amino acids 1–872).

    Techniques: Construct, Activation Assay, Marker, Expressing, Western Blot, Binding Assay